Principles in qPCR and Data analysis

Principles in qPCR and Data analysis, available at $19.99, has an average rating of 3.5, with 8 lectures, based on 2 reviews, and has 23 subscribers.

You will learn about Principles in quantitative PCR Difference in probes Design qPCR program Data analysis and delta delta CT calcutation This course is ideal for individuals who are Students, MSc student, PhD student It is particularly useful for Students, MSc student, PhD student.

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Summary

Title: Principles in qPCR and Data analysis

Price: $19.99

Average Rating: 3.5

Number of Lectures: 8

Number of Published Lectures: 8

Number of Curriculum Items: 8

Number of Published Curriculum Objects: 8

Original Price: $19.99

Quality Status: approved

Status: Live

What You Will Learn

Who Should Attend

Target Audiences

Quantitative polymerase chain reaction (Q-PCR) is a method by which the amount of the PCR product can be determined, in real-time, and is very useful for investigating gene expression. The qPCR method is sometimes also referred to as real-time PCR or depending on the application, quantitative reverse-transcriptase PCR (both of which are abbreviated to RT-PCR, which can be rather confusing). The main advantage of real-time PCR over PCR is that real-time PCR allows you to determine the initial number of copies of template DNA (the amplification target sequence) with accuracy and high sensitivity over a wide dynamic range. Real-time PCR results can either be qualitative (the presence or absence of a sequence) or quantitative (copy number). Quantitative real-time PCR is thus also known as qPCR analysis. In research laboratories, qPCR assays are widely used for the quantitative measurement of gene copy number (gene dosage) in transformed cell lines or the presence of mutant genes. In combination with reverse-transcription PCR (RT-PCR), qPCR assays can be used to precisely quantitate changes in gene expression, for example, an increase or decrease in expression in response to different environmental conditions or drug treatment, by measuring changes in cellular mRNA levels. This course is on real-time PCR, definition, concept, and types of DNA detection directly using DNA binding dye or indirectly using specific fluorescent dye. What is the suitable method for each experiment? How can we perform the qPCR program? Troubleshooting in qPCR. Data analysis of qPCR output.

Course Curriculum

Chapter 1: Introduction

Lecture 1: Introduction to qPCR

Lecture 2: DNA binding Dye

Lecture 3: Probe based qPCR

Lecture 4: Reporter, Quencher, and Passive dyes

Lecture 5: Types of experiement

Lecture 6: qPCR reaction

Lecture 7: Design of qPCR program

Lecture 8: qPCR data analysis

Instructors

Hend Okasha
Dr

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